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il 17a  (Novus Biologicals)


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    Novus Biologicals il 17a
    Il 17a, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tc11+18h10/IL-17%2FIL-17A+Antibody+(TC11-18H10)+-+Azide+and+BSA+Free/pm40887497-110-17-18
    Average 93 stars, based on 6 article reviews
    il 17a - by Bioz Stars, 2026-09
    93/100 stars

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    Article Title: Extensive Histopathological Characterization of Inflamed Bowel in the Dextran Sulfate Sodium Mouse Model with Emphasis on Clinically Relevant Biomarkers and Targets for Drug Development
    Article Snippet: anti-IL-17A , Rat monoclonal, clone TC11-18H10; Novus Biologicals, Littleton, CO, USA , 1:50 , EDTA–Citrate pH 7.8.



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    Effect of helminth exposure on <t>IL-17a</t> protein expression in CD4+ MLN cells from IL-17a lineage reporter mice. MLN cells were isolated from helminth naïve (Control) or H. polygyrus bakeri –infected mice then analyzed by flow cytometry for expression of intracellular IL-17a (A, B) or cultured in the presence of αCD3 for 48h then culture supernatants tested for IL-17a by ELISA (C) . (A) Representative flow cytometry showing gating for CD4+ and IL-17a expression by cells in that gate. (B, C) Data are means ± S.E. from three independent experiments.
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    Effect of helminth exposure on <t>IL-17a</t> protein expression in CD4+ MLN cells from IL-17a lineage reporter mice. MLN cells were isolated from helminth naïve (Control) or H. polygyrus bakeri –infected mice then analyzed by flow cytometry for expression of intracellular IL-17a (A, B) or cultured in the presence of αCD3 for 48h then culture supernatants tested for IL-17a by ELISA (C) . (A) Representative flow cytometry showing gating for CD4+ and IL-17a expression by cells in that gate. (B, C) Data are means ± S.E. from three independent experiments.
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    Effect of helminth exposure on <t>IL-17a</t> protein expression in CD4+ MLN cells from IL-17a lineage reporter mice. MLN cells were isolated from helminth naïve (Control) or H. polygyrus bakeri –infected mice then analyzed by flow cytometry for expression of intracellular IL-17a (A, B) or cultured in the presence of αCD3 for 48h then culture supernatants tested for IL-17a by ELISA (C) . (A) Representative flow cytometry showing gating for CD4+ and IL-17a expression by cells in that gate. (B, C) Data are means ± S.E. from three independent experiments.
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    Effect of helminth exposure on IL-17a protein expression in CD4+ MLN cells from IL-17a lineage reporter mice. MLN cells were isolated from helminth naïve (Control) or H. polygyrus bakeri –infected mice then analyzed by flow cytometry for expression of intracellular IL-17a (A, B) or cultured in the presence of αCD3 for 48h then culture supernatants tested for IL-17a by ELISA (C) . (A) Representative flow cytometry showing gating for CD4+ and IL-17a expression by cells in that gate. (B, C) Data are means ± S.E. from three independent experiments.

    Journal: Frontiers in Immunology

    Article Title: Helminth exposure influences Th17 plasticity, suppressing inflammatory and promoting regulatory activity by Th17 lineage cells

    doi: 10.3389/fimmu.2026.1767639

    Figure Lengend Snippet: Effect of helminth exposure on IL-17a protein expression in CD4+ MLN cells from IL-17a lineage reporter mice. MLN cells were isolated from helminth naïve (Control) or H. polygyrus bakeri –infected mice then analyzed by flow cytometry for expression of intracellular IL-17a (A, B) or cultured in the presence of αCD3 for 48h then culture supernatants tested for IL-17a by ELISA (C) . (A) Representative flow cytometry showing gating for CD4+ and IL-17a expression by cells in that gate. (B, C) Data are means ± S.E. from three independent experiments.

    Article Snippet: XMG 1.2 (αIFN-γ, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA), 2A5 (αIL-10, ATCC, CRL-2444), TC11-18H10.1 (αIL-17a, BioLegend, San Diego, CA United States of America), and C16.5 (αIL-12p40, hybridoma: a gift from Dr. G. Trinchieri, (Wistar Institute of Anatomy and Biology, Philadelphia, PA).

    Techniques: Expressing, Isolation, Control, Infection, Flow Cytometry, Cell Culture, Enzyme-linked Immunosorbent Assay

    Helminth exposure effects frequency of CD4+ eYFP + cells and the level of IL-17a expression in MLN cells from IL-17a lineage reporter mice. MLN cells were isolated from helminth naïve (Control) or H. polygyrus bakeri -infected mice then analyzed by flow cytometry for expression of both eYFP and intracellular IL17a. (A) Representative flow cytometry showing gating for CD4+ eYFP+ cells and IL-17a expression by cells in that gate. (B) and (C) Data are means ± S.E. from three independent experiments.

    Journal: Frontiers in Immunology

    Article Title: Helminth exposure influences Th17 plasticity, suppressing inflammatory and promoting regulatory activity by Th17 lineage cells

    doi: 10.3389/fimmu.2026.1767639

    Figure Lengend Snippet: Helminth exposure effects frequency of CD4+ eYFP + cells and the level of IL-17a expression in MLN cells from IL-17a lineage reporter mice. MLN cells were isolated from helminth naïve (Control) or H. polygyrus bakeri -infected mice then analyzed by flow cytometry for expression of both eYFP and intracellular IL17a. (A) Representative flow cytometry showing gating for CD4+ eYFP+ cells and IL-17a expression by cells in that gate. (B) and (C) Data are means ± S.E. from three independent experiments.

    Article Snippet: XMG 1.2 (αIFN-γ, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA), 2A5 (αIL-10, ATCC, CRL-2444), TC11-18H10.1 (αIL-17a, BioLegend, San Diego, CA United States of America), and C16.5 (αIL-12p40, hybridoma: a gift from Dr. G. Trinchieri, (Wistar Institute of Anatomy and Biology, Philadelphia, PA).

    Techniques: Expressing, Isolation, Control, Infection, Flow Cytometry

    Co-transfer of Th17 lineage MLN cells from mice exposed to H. polygyrus bakeri alters LPMC cytokine expression in mice with T cell transfer colitis. LPMC were isolated from colons of Rag-1 −/− mice reconstituted with CD25neg splenic T cells co-transferred with flow-cytometry isolated MLN eYFP+ T cells from helminth naïve (open box) or H. polygyrus bakeri -infected (gray box) IL-17 reporter mice, stimulated with anti-CD3 (IFN-γ,IL-17a, IL-10, IL-4) or LPS (IL-12p40) and cultured for 48h. Culture supernatants were analyzed for cytokines by ELISA. Data are means ± SE from three independent experiments.

    Journal: Frontiers in Immunology

    Article Title: Helminth exposure influences Th17 plasticity, suppressing inflammatory and promoting regulatory activity by Th17 lineage cells

    doi: 10.3389/fimmu.2026.1767639

    Figure Lengend Snippet: Co-transfer of Th17 lineage MLN cells from mice exposed to H. polygyrus bakeri alters LPMC cytokine expression in mice with T cell transfer colitis. LPMC were isolated from colons of Rag-1 −/− mice reconstituted with CD25neg splenic T cells co-transferred with flow-cytometry isolated MLN eYFP+ T cells from helminth naïve (open box) or H. polygyrus bakeri -infected (gray box) IL-17 reporter mice, stimulated with anti-CD3 (IFN-γ,IL-17a, IL-10, IL-4) or LPS (IL-12p40) and cultured for 48h. Culture supernatants were analyzed for cytokines by ELISA. Data are means ± SE from three independent experiments.

    Article Snippet: XMG 1.2 (αIFN-γ, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA), 2A5 (αIL-10, ATCC, CRL-2444), TC11-18H10.1 (αIL-17a, BioLegend, San Diego, CA United States of America), and C16.5 (αIL-12p40, hybridoma: a gift from Dr. G. Trinchieri, (Wistar Institute of Anatomy and Biology, Philadelphia, PA).

    Techniques: Expressing, Isolation, Flow Cytometry, Infection, Cell Culture, Enzyme-linked Immunosorbent Assay